Lightsheet Conference
10th Anniversary Light Sheet Fluorescence Microscopy Conference
12 - 15 August, 2018 | Dresden, Germany

About the Conference

Light sheet microscopy has emerged in the last 15 years as a powerful tool to interrogate living biological systems with unprecedented imaging speeds for as long as it takes. Large, fixed, cleared or expanded biological samples can be imaged with high resolution in a fraction of time compared to conventional microscopy approaches. When combined, the spatial coverage and temporal resolution leads to in toto recording of complex biological phenomena.

Light sheets opened entirely new windows into the inner workings of cells and organisms and re-invigorated several research fields including developmental and cell biology, physiology, neurobiology and biodiversity research. This conference marks the 10th Anniversary of Light Sheet Fluorescence Microscopy (LSFM) meetings that started in 2009 in Dresden. It will bring together the technology developers and users of light sheets, showcase the latest light sheet set-ups and how to best deploy them for the benefit of the biological research community.

A unique feature of the conference is that it immediately follows the 3rd EMBO practical course on light sheet microscopy held in Dresden between August 2nd and 11th 2018. This course teaches light sheet microscopy to the next generation of scientists who will present the results of their light sheet experiments at the conference. Additionally, a full range of commercial light sheet microscopy hardware used at the course will be demonstrated at the conference.



Registration Deadline

July 6st, 2018

Abstract Submission Deadline

July 6th, 2018

Our Amazing Speakers


Francesca Cella Zanacchi

Italian Institute of Technology, Genoa, IT

Christian Conrad

Deutsches Krebsforschungszentrum Heidelberg (DKFZ), DE

Reto Fiolka

UT Southwestern Medical Center, Dallas, USA

Scott E. Fraser

University of Southern California, Los Angeles, USA

Rainer Heintzmann

Institute of Physical Chemistry, Jena University, DE

Elizabeth Hillman

Department of Biomedical Engineering Columbia University, USA

Lars Hufnagel

EMBL, Heidelberg, DE

Jan Huisken

Morgridge Institute for Research, Madison, Wisconsin, USA

Florian Jug

MPI-CBG, Dresden, DE

Srigokul Upadhyayula

Boston Children's Hospital, Harvard Medical School, Boston, USA

Philippe Laissue

University of Essex, GB

Corinne Lorenzo

Paul Sabatier University, Toulouse, FR

Katie McDole

Janelia Research Campus, USA

Gene Myers

MPI-CBG, Dresden, DE

Caren Norden

MPI-CBG, Dresden, DE

Nadine Peyrieras

CNRS Laboratory USR3695 BioEmergences, FR

Peter Santi

University of Minnesota, Minneapolis, USA

Ernst Stelzer

Goethe University, Frankfurt am Main, DE

Rita Strack

Nature Methods, New York, USA

Willy Supatto

Ecole Polytechnique, Paris Saclay University, FR

James Swoger

EMBL Barcelona, SP

Raju Tomer

Biological Sciences, Columbia University, USA

Hiroki Ueda

RIKEN Quantitative Biology Center, Kobe, JP

Daniel von Wangenheim

School of Biosciences, University of Nottingham, GB

Programme

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Day
Time / Session
Speaker / Title

Day 1 - Sunday, August 12

16:00 - 18:00

Registration


18:00 - 18:05

Welcome from the Managing Director of MPI-CBG

Gene Myers


18:05 - 18:30

Retrospect on 10 years of LSFM meetings

Pavel Tomancak, Emmanuel Reynaud


18:30 -

Reception at MPI-CBG


Day 2 - Monday, August 13

09:00 - 10:30

Session 1: Light sheet hardware I


09:00 - 09:15

Invited talk 1

Ernst Stelzer

Resolution and applications of LSFM in the life sciences


09:15 - 09:30

Invited talk 2

Elizabeth Hillman

Applications and extensions of SCAPE microscopy


09:30 - 09:45

Invited talk 3

Scott Fraser

Adding Dimensions to Light Sheet Imaging


09:45 - 10:00

Selected talk 1

Petr Strnad

Dual illumination inverted light sheet microscope for long term live imaging


10:00 - 10:15

Selected talk 2

Fabian Voigt

The mesoSPIM initiative – open-source light-sheet microscopes for imaging in cleared tissue


10:15 - 10:30

Selected talk 3

Jonathan Nylk

Single- and multi-photon shaped illumination for light-sheet fluorescence microscopy


10:30 - 11:15

Coffee break


11:15 - 12:45

Session 2: Light sheet hardware II


11:15 - 11:30

Invited talk 4

Jan Huisken

Flamingos for you and me


11:30 - 11:45

Invited talk 5

Rainer Heintzmann

Lightwedge and Lightsheet-Raman Microscopy


11:45 - 12:00

Invited talk 6

Rita Strack

Light sheet microscopy in Nature Methods


12:00 - 12:15

Selected talk 4

Hans-Ulrich Dodt

Fast nearly isotropic imaging of large samples with light sheets beyond the diffraction limit


12:15 - 12:30

Selected talk 5

Moritz Kreysing

Focused-light-induced cytoplasmic streaming (FLUCS). A new paradigm to probe the physiology on intracellular transport.


12:30 - 12:45

Selected talk 6

Francesca Cella Zanacchi

High resolution and quantitative super-resolution microscopy: from cells to tissue imaging


12:45 - 14:00

Lunch in the MPI-CBG canteen


14:00 - 15:00

Techno-bites: Short talks from companies


14:00 - 14:05

Techno-bite 1

ZEISS

Lightsheet Z.1 – The Original.


14:05 - 14:10

Techno-bite 2

LaVision BioTec

Cleared tissue imaging


14:10 - 14:15

Techno-bite 3

Applied Scientific Instrumentation

ASI makes DIY light sheet easy


14:15 - 14:20

Techno-bite 4

Intelligent Imaging Innovations GmbH

Imaging Life with 3i LightSheet Systems


14:20 - 14:25

Techno-bite 5

Leica

SP8 DLS – Leica`s Lightsheet on a Confocal at a glance


14:25 - 14:30

Techno-bite 6

Luxendo

Luxendo Light-Sheet Microscopes: Seeing Life from a Different Angle


14:30 - 14:35

Techno-bite 7

PhaseView

Alpha3, a new generation of light sheet microscope


14:35 - 14:40

Techno-bite 8

ANDOR/Bitplane

Quantitative 3D and 4D image analysis for Light Sheet Microscopy in Imaris


14:40 - 14:45

Techno-bite 9

PCO

To cool or not to cool? How cooling influences the performance of sCMOS-cameras


14:45 - 14:50

Techno-bite 10

Hamamatsu

Extended Focus Device


14:50 - 14:55

Techno-bite 11

Acquifer

Large Image Data Solutions by Acquifer


15:00 - 17:00

Company demos and poster session I


17:00 - 18:30

Session 3: Image analysis of light sheet data


17:00 - 17:15

Invited talk 7

Gene Myers

Different Rigs for Different Gigs


17:15 - 17:30

Invited talk 8

Lars Hufnagel

Bioimaging information: combining light sheet imaging with single cell transcriptomics


17:30 - 17:45

Invited talk 9

Florian Jug

Content-Aware Image Restoration and Quantitative Downstream Analysis


17:45 - 18:00

Selected talk 7

Bevan Cheeseman

Towards next generation imaging pipelines using the Adaptive Particle Representation.


18:00 - 18:15

Selected talk 8

Kaushikaram Subramanian

Light propagation in retinal tissue and in silico


18:15 - 18:30

Selected talk 9

David Hörl

BigStitcher: Reconstructing high-resolution image datasets of cleared and expanded samples


18:30 - 20:00

Dinner at MPI-CBG


20:00 -

Poster session II (with drinks)


Day 3 - Tuesday, August 14

09:00 - 10:30

Session 4: Sample preparation, clearing and expansion


09:00 - 09:15

Selected talk 11

Tobias Pietzsch

Mastodon -- a large-scale track-editing framework for light sheet data


09:15 - 09:30

Selected talk 12

Carsten Wolff

Cellular basis of limb morphogenesis: lessons from the crustacean Parhyale hawaiensis


09:30 - 09:45

Invited talk 10

Hiroki Ueda

Whole-body/organ Cell Profiling by a Hydrophilic Tissue-clearing method, CUBIC


09:45 - 10:00

Invited talk 11

Jan Peychl

12 years with Light Sheet Microscopy in core imaging facilities on Dresden campus


10:00 - 10:15

Invited talk 12

Jim Swoger

Attenuation Artifacts in LSFM Corrected by OPTiSPIM


10:15 - 10:30

Selected talk 10

Stephan Daetwyler

Multi-sample SPIM image acquisition, processing and analysis of embryonic zebrafish vasculature


10:30 - 11:15

Coffee break


11:15 - 12:45

Session 5: Neurobiology applications of light sheet microscopy


11:15 - 11:30

Invited talk 13

Raju Tomer

Light sheet theta microscopy for quantitative imaging of large cleared samples.


11:30 - 11:45

Invited talk 14

Caren Norden

Photoreceptors catching the light(sheet): Fate, movements and lamination


11:45 - 12:00

Selected talk 13

Marija Matejcic

A non-cell autonomous actin redistribution enables isotropic retinal growth


12:00 - 12:15

Selected talk 14

Nikita Vladimirov

Brainwide optical circuit interrogation guided by online analysis of neuronal function


12:15 - 12:30

Selected talk 15

Emilio J Gualda

High-Throughput LSFM Imaging Of 3D-3-Culture Models To Unveil Macrophage Plasticity In The Tumour Microenvironment


12:30 - 12:45

Selected talk 16

Jonathan Taylor

Hybrid Optically-Gated Light Sheet Microscopy Allows Long-term Timelapse Imaging in the Developing Zebrafish Heart


12:45 - 14:00

Lunch in the MPI-CBG canteen


14:00 - 16:00

Company demos and poster session III


16:00 - 16:45

Presentations of EMBO practical course teams (4 teams 10 minutes each)


16:00 - 16:10

EMBO course

Team 1

Ernstigators


16:10 - 16:20

EMBO course

Team 2

Guide Stars


16:20 - 16:30

EMBO course

Team 3

Light Benders


16:30 - 16:40

EMBO course

Team 4

You SPIM Me (Baby)


17:00 - 18:30

Session 6: Cell biology applications of light sheet microcopy


17:00 - 17:15

Invited talk 15

Srigokul Upadhyayula

In Vivo Cellular Dynamics imaged using Adaptive Optics Lattice Light Sheet Microscopy


17:15 - 17:30

Invited talk 16

Christian Conrad

Imaging organoids in diSPIM


17:30 - 17:45

Invited talk 17

Reto Fiolka

Light-sheet microscopy at high spatiotemporal resolution


17:45 - 18:00

Selected talk

Miroslav Ovecka

Light sheet fluorescence microscopy in plant developmental imaging


18:00 - 18:15

Selected talk 17

Lars Hubatsch

Interplay between cell size and cell polarity


18:15 - 18:30

Selected talk 18

Chris Dunsby

High content 3-D light sheet microscopy in 96 and 384-well plate formats applied to study cancer cell size, and invasiveness and morphology in 3D matrices


18:30

Bus transfer from MPI-CBG


19:00 - 23:00

Conference dinner


Day 4 - Wednesday, August 15

09:00 - 10:30

Session 7: Developmental biology applications of light sheet microscopy


09:00 - 09:15

Invited talk 19

Nadine Peyrieras

3D+time imaging of developing embryos for the reconstruction of their cell lineage


09:15 - 09:30

Invited talk 20

Willy Supatto

Exploring a new contrast mechanism for light-sheet microscopy: fast harmonic generation imaging


09:30 - 09:45

Invited talk 21

Katie McDole

Imaging and dynamic analysis of the post-implantation mouse embryo at the cellular level using light-sheet microscopy


09:45 - 10:00

Selected talk 19

Stefan Muenster

The Physics of Blastoderm Flow during Early Gastrulation of Tribolium castaneum


10:00 - 10:15

Selected talk 20

Robert Haase

Smart adaptive multi-sample imaging for multi-view light sheet microscopy – when the microscope decides what to look at


10:15 - 10:30

Selected talk 21

Vikas Trivedi

Imaging axial emergence though self-organization in embryonic organoids.


10:30 - 11:15

Coffee break


11:15 - 12:45

Session 8: Miscellaneous applications of light sheet microscopy


11:15 - 11:30

Invited talk 22

Philippe Laissue

Imaging photosensitive reef-building corals


11:30 - 11:45

Invited talk 23

Daniel von Wangenheim

Root organogenesis is biased towards the availability of water


11:45 - 12:00

Invited talk 24

Douglas Shepherd

Molecular mapping of developmental disease progression using HiTS-FAST


12:00 - 12:15

Selected talk 22

Joerg Mansfeld

Conditional control of fluorescent protein degradation by an auxin-dependent nanobody


12:15 - 12:30

Selected talk 23

Raghuveer Parthasarathy

Imaging Intestinal Invasion by Vibrio cholerae, and other Stories from the Gut


12:30 -12:45

Selected talk 24

Peter Santi

Macro LSFM with sTSLIM (scanning Thin-Sheet Laser Microscopy)


12:45 - 13:00

Closing of the workshop


13:00 - 14:00

Lunch in the MPI-CBG canteen


Registration


Student/Postdoc/Academic

250 EUR

Industry

600 EUR

Registration Deadline

July 6st, 2018


Abstract Submission Deadline

July 6th, 2018

Contact Us


Christina Kuss
+49-351-210-2805
Sandy Schneider
+49-351-210-2756

Organizers




Pavel Tomancak

@PavelTomancak

Max Planck Institute of Molecular Cell Biology & Genetics, Dresden, DE

Stephan Preibisch

@preibischs

Max-Delbrück-Centrum für Molekulare Medizin (MDC), Berlin, DE

Emmanuel Reynaud

@ReynaudEmmanuel

Conway Institute of Biomolecular and Biomedical Science, Dublin, IE

Location


Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstr. 108
D-01307 Dresden
Germany

Accommodation

Participants are expected to book and pay their own accommodation and travel expenses. Some rooms have been reserved at our partner hotels, allowing participants to choose a suitable hotel at a discounted rate.

Gewandhaus Dresden

Ringstraße 1

01067 Dresden

info@gewandhaus-hotel.de

Single room (incl. breakfast) 72 EUR

Available until 13 July

Hotel Am Blauen Wunder

Loschwitzer Straße 48

01309 Dresden

reservierung@habw.de

Single room (incl. breakfast) 78 EUR

Available until 30 June

Hotel Pension Andreas

Prellerstraße 18

01309 Dresden

reservierung@hotel-pension-andreas.de

Single room (incl. breakfast) 69 EUR

Double room (incl. breakfast) 89 EUR

Available until 31 May

Steigenberger Hotel de Saxe

Neumarkt 9

01067 Dresden

desaxe-dresden@steigenberger.de

Single room (incl. breakfast) 90 EUR

Double room (incl. breakfast) 149 EUR

Available until 15 June

Please use our code MPIAugust2018 when you contact one of the hotels to ensure you receive the discounted rate.

In addition, please note that the City of Dresden has effected a citywide lodging tax for non-business travellers (“Beherbergungssteuer”). In order to be exempt from this taxation you need to fill in this form and present it to the hotel upon check-in.

More information on this can be found here: https://www.dresden.de/media/pdf/infoblaetter/2017-10_Infoblatt_Gaeste_EN.pdf

Transportation

Via Plane

Dresden has a relatively small airport connected by Lufthansa to major German hubs such as Frankfurt, Munich and Düsseldorf. There is also an Air France flight from London.

Berlin Schoenefeld International airport is about 172 km from Dresden, easily reachable by regular trains or busses.

Vaclav Havel Airport Prague in the Czech Republic is another option, 160 km away, also connected by trains (about every two hours) and busses.

From the Dresden Airport, please take the train S2 available every 30 min to the train station Bahnhof-Neustadt. Then take the tram 6, direction Niedersedlitz, to the station Königsheimplatz. Cross the street and walk along Schubertstraße until you come to Pfotenhauerstraße and turn left. Our institute is at Pfotenhauerstraße 108. It is a green and blue building. It takes about 40 minutes.

Via Train

Dresden is easily reachable by train. There are two train stations in Dresden: Hauptbahnhof and Bahnhof-Neustadt.

From the main train station [Hauptbahnhof] please take the tram 10, direction Striesen, to the station Fetscherplatz. Then take the tram 12, direction Striesen, to the station Königsheimplatz. Cross the street and walk along Schubertstraße until you come to Pfotenhauerstraße and turn left. Our institute is at Pfotenhauerstraße 108. It is a green and blue building. It takes about 20 minutes.

You can also use tram 3 (Wilder Mann) or 7 (Weixdorf) and change at Pirnaischer Platz to bus 62 (Johannstadt). The terminal stop is right opposite of the Institute. It takes about 23 minutes.

From the train station Bahnhof-Neustadt take tram 6, direction Niedersedlitz, to the station Königsheimplatz. Cross the street and walk along Schubertstraße till you come to Pfotenhauerstraße and turn left. Our institute is at Pfotenhauerstraße 108. It takes about 15 minutes.

Reaching the Institute via tram or bus

Take tram 6 or 12 to the station Königsheimplatz. Walk along Schubertstraße until you come to Pfotenhauerstraße and turn left.

Alternatively, take bus 62 to Johannstadt or 64 to stop Pfotenhauerstraße.

To check the schedule of public transport use this website.

Via Car

From Berlin (north)

Leave A4 at the exit Dresden-Hellerau, which will take you onto the B 170 heading towards the city center (Zentrum). Stay on the B 170 for about 5 km until it reaches the intersection "Carolaplatz". At the next intersection turn right onto the "Albertbrücke" bridge. From now on follow the signs "Uniklinikum" to the medical clinic. Once over the bridge, you have to drive a circle in order to turn to the left, which means taking the second street to the right, then turn to the right and to the right again onto "Käthe-Kollwitz-Ufer." Follow "Käthe-Kollwitz-Ufer" and turn right into "Fetscherstraße." At the first intersection turn left into "Pfotenhauerstraße." Our institute is on the right.

From Munich/Frankfurt/Leipzig (south)

Leave Autobahn A4 at the exit Dresden-Altstadt, which will take you onto the B 6. Follow the signs to the city center (Zentrum) and stay on the B 6 for about 4 km until an intersection with a Turkish Mosque on the left side. Still following the signs to the city center (Zentrum), turn left and pass the Mosque. Follow the road which turns to the right and goes under the railway bridge. This leads you to "Uferstrasse" which takes two sharp bends (passing Semperoper opera house). Now you are on the "Terrassenufer" which takes you parallel to the river Elbe. Stay on this road following the sign "Uniklinikum." The street merges to "Käthe-Kollwitz-Ufer." Follow "Käthe-Kollwitz-Ufer" and turn right into "Fetscherstrasse." At the first intersection turn left into "Pfotenhauerstrasse." Our institute is on the right

From Prague

Follow the highway D8 going north from the Prague circle towards Aussig (Usti nad Labem) or Teplitz (Teplice) and Dresden. The highway is interrupted in the middle by the scenic Ceske Stredohori mountain for about 20 km (narrow road, follow signs for Dresden). The trip takes about two hours.

About the Area

The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), founded in 1998, is one of 80 institutes of the Max Planck Society, an independent non-profit organization in Germany.

In recent years Dresden has developed at a tremendous pace into one of the leading scientific centers in the areas of cell and developmental biology, biomedicine, biophysics and bioengineering. Dresden is characterized by a vibrant international community of researchers that perform cross-disciplinary research at the highest level in state-of-the-art research centers at the Technische Universität Dresden (TUD), the Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), the Max Planck Institute for the Physics of Complex Systems (MPI-PKS) and several Leibniz and Fraunhofer Institutes.

Dresden is considered one of the most beautiful cities of Germany. It is located in the east of Germany in the valley of the river Elbe. Dresden is about 2.5 hrs away from Berlin as well as Prague. Being the capital of Saxony it has been a center of political and cultural life for centuries, and its skyline with the Zwinger, the Green Vault, the Painting Gallery, the castle, the Semper Opera, the Court Chapel, Taschenberg Palace, and the recently rebuilt Frauenkirche are world-famous. But the city is not only famous for its baroque architecture and beautiful surrounding landscapes. Dresden has evolved into a vivid and international scientific and industrial center and is one of the most outstanding locations for research and science in Germany with emphasis on biotechnology, nano- and microelectronics. In appreciation of this dynamic development, Dresden has been awarded the “City of Science” in 2006.

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