mClub
We are people who do microscopy or want to learn about it. Our club is very diverse: physicists, biologists, computer scientists, and DIY geeks. We meet monthly and talk about ray and wave optics, old and new microscopy methods, fluorescent probes, cameras, objectives, etc. Volunteers and guest speakers are welcome!
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Upcoming meetings:
TBAPast meetings:
Inna Kviatovsky (Ramelow Lab, HU) “Microscopy with undetected photons in the mid-IR”
Philipp Rauch (Lumicks) “Real-time single-molecule investigation of dynamic biological processes using correlative optical tweezers and fluorescence microscopy: From structure to function.”
Daniel von Wangenheim (3i) “Advanced microscopy by 3i - Intelligent Imaging Innovations.”
Maximilian Hoffmann (Judkewitz Lab) “Oblique plane microscopy for large field of views.” Talk + DEMO of the brand new microscope!
Ella de Gaulejac (Reber Lab) “Combining Expansion Microscopy and STED Microscopy: current tools towards super resolution in fluorescence microscopy”
Abin Biswas (Reber Lab) “Optical Diffraction Tomography: A quantitative label-free 3D imaging tool”
Simone Reber “How Complexity Arises from Molecular Interactions”
Tobias Kletter (Reber Lab) “Teaching machines to do all the work: using adaptive feedback microscopy on complex samples”
Stephan Prebisch: Simulating light propagation in tissues using raytracing.
Nikita Vladimirov: Highlights from Swiss Light-sheet Microscopy Workshop.
Martin Fritsch and Svenja Kunerth (Leica Microsystems). Lightning and Thunder adaptive image extraction and deconvolution.
Marie Walde (Woehler Lab). Confocal detectors.
Nikita Vladimirov (Preibisch Lab). Deconvolution for dummies.
Friedrich Preusser (Preibisch Lab). FISHing under microscopes: An overview of recent techniques for spatial transcriptomics.
Journal club (F. Preusser): DNA microscopy: Optics-free spatio-genetic imaging by a stand-alone chemical reaction. J.A. Weinstein, A.Regev, F.Zhang.
Nikita Vladimirov (Preibisch Lab). Metrology hands-on demo: how to measure hair width and other tiny things with micron precision without a microscope.
Demo an ultra-compact microscope for confocal/SIM/TIRF/single molecule imaging, Nanoimager.
Christopher Schmied, FMP. Digital images and their analysis.
Selma E. Anton, Lohse Lab. Lasers: where do the colors come from, what can they be used for and everything else you need to know.
Nikita Vladimirov, Preibisch Lab. Imaging, brain mapping, and messing with neuronal networks in zebrafish brain (journal club).
Elisabeth Baumann, Gerhardt Lab, MDC.
Creating sound with light: The principle and applications of Photoacoustic Microscopy.
Marie Walde, Woehler Lab, MDC.
Co-localization analysis of fluorescence images: Overview & recent development.
Journal Club: Mapping molecular assemblies with fluorescence microscopy and object-based spatial statistics, Lagache et al, Nat. Comm. 2018.
Konstantin Grohmann, Advanced Light Microscopy platform, MDC.
Examining the performance of a fluorescence microscope.
Slides
Benjamin Judkewitz, head of the Bioimaging and Neurophotonics Lab at Charité.
Deep imaging with time-reversed light.
An unresolved mystery of physics: the Double-Slit Experiment. Watching The Quantum Experiment that Broke Reality | Space Time | PBS Digital Studios and the classics, Richard Feynman’s Lecture on Electron Two-Slit Experiment
Sensors and cameras. Discussion of Nico Stuurman’s online lectures from ibiology.org
Martin Lehmann, Core Facility Cellular Imaging FMP
Introduction to fluorescent probes in microscopy. Quantitative assessment of fluorescent proteins. (Cranfill et al., Nat. Methods, 2016).
Slides
Nikita Vladimirov, Preibisch Lab
Autofocus in microscopy and photography. RAPID: Real-time image-based autofocus for all wide-field optical microscopy systems. (Silvestri et al, 2017).
Slides
Varun Kapoor, Preibisch Lab
Maxwell equations. Slides
Nikita Vladimirov, Preibisch Lab
Gaussian and Bessel beams. Slides
Marie Walde, Woehler Lab
Optical resolution and diffraction. Slides
Nikita Vladimirov, Preibisch Lab
Geometrical optics. Slides